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one hybrid library screening system  (TaKaRa)


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    TaKaRa one hybrid library screening system
    One Hybrid Library Screening System, supplied by TaKaRa, used in various techniques. Bioz Stars score: 98/100, based on 1220 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/one+hybrid+system/Matchmaker+Gold+Yeast+One-Hybrid+Library+Screening+System/10__1016_slash_j__indcrop__2026__123372-72-13-17
    Average 98 stars, based on 1220 article reviews
    one hybrid library screening system - by Bioz Stars, 2026-09
    98/100 stars

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    other:

    Article Title: H₂S mitigates chilling injury by downregulating PpbHLH113 to enhance phenylpropanoid biosynthesis in peach fruit
    Article Snippet: Postharvest chilling injury (CI) severely limits peach fruit quality.. Hydrogen sulfide (H2S) has shown potential in mitigating CI, while its underlying transcriptional regulation mechanism remains insufficiently characterized.. This study revealed that H2S treatment effectively mitigated CI by promoting both the enzymatic activities and transcript levels of key phenylpropanoid biosynthesis enzymes (PAL, C4H, 4CL, and CHI), accompanied by increased accumulation of total phenolics and flavonoids.

    Article Title: Anthocyanin accumulation differences in European pears caused by Phytochrome-interacting factor 3 (PcPIF3) promoter mutations under UV-B
    Article Snippet: Y1H assays were conducted following the manufacturer's protocol, utilizing the Matchmaker Gold Yeast One-Hybrid System (Clontech, Mountain View, CA, USA).

    Article Title: PdTP1 regulates the biotransformation of limonene to α-terpineol by targeting cytochrome P450 genes in Penicillium digitatum
    Article Snippet: As an important functional monoterpene, α-terpineol can be generated through biotransformation.. The transcription factor PdTP1 has been previously validated for its beneficial role in α-terpineol production via limonene biotransformation in Penicillium digitatum DSM 62840.. However, the mechanistic basis of PdTP1-mediated transcriptional regulation remained elusive.

    Article Title: JsMYB170 modulates flavonols biosynthesis through facilitation of JsFLS1/5 expression in pellicle of Juglans sigillata Dode.
    Article Snippet: J. sigillata produces abundant flavonols such as rutin and myricetin in pellicle, which provide various benefits to human health.. However, the transcriptional regulation of flavonols biosynthesis in pellicle of J. sigillata remains unclear.. Our study showed that the JsMYB170 is a transcriptional activator involved in the biosynthesis of flavonols in J. sigillata.

    Article Title: Red light pretreatment promotes esters biosynthesis by activating the CmBBX7/14-CmLOX18-CmAAT1 module in postharvest oriental melon at low temperature
    Article Snippet: Combining light quality regulation with postharvest low-temperature storage holds promise for mitigating postharvest aroma loss in fruits.. However, the molecular mechanism by which light quality modulates postharvest aroma biosynthesis remains unclear.. In this study, ‘Bali Xiang’ oriental melon fruit at the early stage of ethylene release were selected as experimental material and subjected to red light (RLT) or blue light (BLT) pretreatment prior to low-temperature storage.

    Article Title: Telomere-to-telomere genome assemblies and population resequencing of diploid and allotetraploid peanut varieties.
    Article Snippet: Protein–DNA interactions were tested using a Matchmaker Gold Yeast One-Hybrid System (Takara Bio/Clontech, catalogue number 630491).

    Binding Assay:

    Article Title: Multiomics Analysis across the Life Cycle Identifies Zn 2 Cys 6 _61 as a Target for Enhancing Triterpenoid Production in Ganoderma lucidum
    Article Snippet: .. Yeast one-hybrid (Y1H) assays were performed by using the MATCHMAKER Gold Yeast One-Hybrid system (630491, Clontech) to examine the direct binding of Zn 2 Cys 6 _61 to the promoter of SQS . .. Promoter fragments of SQS containing G-box elements were amplified using primers listed in Table S10 and cloned into the pAbAi vector using the In-Fusion HD Cloning Kit (639648, Takara Bio).

    Article Title: Multiomics Analysis across the Life Cycle Identifies Zn 2 Cys 6 _61 as a Target for Enhancing Triterpenoid Production in Ganoderma lucidum .
    Article Snippet: .. Yeast one-hybrid (Y1H) assays were performed by using the MATCHMAKER Gold Yeast One-Hybrid system (630491, Clontech) to examine the direct binding of Zn2Cys6_61 to the promoter of SQS. .. Promoter fragments of SQS containing G-box elements were amplified using primers listed in Table S10 and cloned into the pAbAi vector using the In-Fusion HD Cloning Kit (639648, Takara Bio).



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